About 3DCellCulture

FAQ: The most Frequently Asked Questions about 3dcellculture.com

1) What are your criteria for citations to be included in the collection? What’s NOT included?


We have made our best efforts to find and include ALL relevant, peer-reviewed literature citations featuring three dimensional (3D) cell culture techniques, and ONLY relevant, peer-reviewed literature citations featuring three dimensional (3D) cell culture techniques.


We’ve outlined our strategy and method below. 


Criterion which must be met for citations to be included in the 3dcellculture.com collection:
As a result, the following are excluded from the 3dcellculture.com collection:
Why? (Remember, the focus of the 3dcellculture.com website is 3D and cell culture …)
  • Methods of original research papers must use both cells and matrix materials
  • Papers featuring cells cultured on typical flat surfaces (e.g. historic 2D cell culture)
  • Papers featuring only a proposed new scaffold material / no use with cells
  • Such papers do not feature cells cultured in 3D
  • Such papers do not utilize cell culture

*Exception to above: Techniques which demonstrably generate matrix in situ; e.g. pellet or rotary methods


* The lack of matrix is a temporal technicality; it has been shown that these techniques result in the formation of matrix materials over time

  • Methods of original research papers must utilize in vitro techniques
  • Papers featuring only in vivo implantation of construct designed to attract cells from surrounding tissue
  • Such papers do not utilize cell culture
  • Methods of original research papers must utilize in vitro analysis or characterization of cell/matrix construct
  • Papers featuring cell seeded but unanalyzed cell/matrix constructs later implanted in vivo

 

  • Such papers do not provide any information about cell culture; i.e. it is a means but not an end
  • Review papers describing a topic expressly targeted towards 3D cell culture or tissue engineering, even those which may feature only a cell focus or only a material type
  • Other review papers lacking a 3D cell culture or tissue engineering focus
  • Such reviews do not provide information about cell culture
  • Papers must feature eukaryotic cells
  • Papers featuring bacterial cultures
  • Lack of relevance to website focus
  • Papers must feature cells having mammalian or avian origin
  • Papers featuring plant, mollusk, insects, spiders, fish or amphibian cells
  • Lack of relevance to website focus
  • Matrix or scaffold material must have a minimum thickness of 50 µm (micrometers) within which cells can be situated
  • Papers featuring typical surface coatings or films
  • Assuming an average minimum cell dimension of 10 µm (micrometers), very few if any cells in such papers are in a 3D environment
*Exception to above: Surfaces which demonstrably induce spheroid or aggregate formation of cultured cells

 


  • Cells in aggregates or spheroids are in a 3D environment; see in situ matrix generation exception above
  • Must be peer-reviewed
  • Conference abstracts or proceedings
  • Typically such short papers lack detail on materials and methods and cannot be independently replicated
  • Citation and abstract must be available in English language
  • Citations not available in English
  • Inability to characterize or utilize content due to language limitations of both web site staff and majority audience



Admittedly, some of these criteria are subjective. Disagree with where we’ve drawn the lines? Let us know. We’re happy to engage in intelligent discussion on a topic we’re passionate about.


2) I authored or know of a paper that fits the criteria but is not included. How can I submit it?


Cut and paste the citation information into a text email, and send it to us. We’ll gladly add citations that we have somehow missed.


3) I found a mistake in the way one of the citations was indexed.


Although we do our best to check every one of the several thousand papers we feature, this occasionally happens. Let us know which citation and what the error is and we’ll fix it immediately for the benefit of future searchers.